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Cular studies on asbestos and ROS effects on TUSC2 expression, was involved in the analysis of microarray data and their molecular confirmation, processed all the data and wrote the manuscript, SI carried out mesothelioma genomic and transcriptomic database analyses, was involved in molecular experimentations and helped to draft two chapters of the manuscript, LP performed the analysis and scoring
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Protein-A beads were washed 36 with 1 ml NP-40 lysis buffer, and then pre-incubated with antibody for 2 h before adding to 1 ml of lysate (1 mg/ml protein). Immunoprecipitations were performed overnight at 4uC.Creation of GST-fusion ProteinsUsing the pCaSpeR-4 Roc constructs as template [25], PCR products were made using primers that added EcoR1 sites on either side, and the product was then clone
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