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Ing assayed for changes in cell viability.ELISA GIST882 cells (3 ?106) were treated with imatinib for 24 or 48 hours. After treatment, the conditioned medium was collected and briefly centrifuged to remove the floating cells and cellular debris. Aliquots (50 L) of the supernatant were analyzed for the presence of IGFBP3 using the Human IGFBP3 Quantikine ELISA kit (#DGB300) from R D Systems (Minnea
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