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Product ended up being watered down 1/30 within PCR quality normal water. Picky amplifications had been incubated with all the subsequent cycle: 95��C (9?min), 94��C (30?s), 65��C (30?s) (reduce by 1��C per period), 72��C (2?min), come back to step # 2 along with duplicate 7 far more times, 94��C (30?s), 56��C (30?s), 72��C (2?min), return to step 6 and duplicate 30 instances, 72��C (5?min) as wel
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