Articlebase Social Networking Place - Protein-A beads were washed 36 with 1 ml NP-40 lysis buffer, and then http://www.articlebase.co.uk/story.php?title=protein-a-beads-were-washed-36-with-1-ml-np-40-lysis-buffer-and-then-17 Protein-A beads were washed 36 with 1 ml NP-40 lysis buffer, and then pre-incubated with antibody for 2 h before adding to 1 ml of lysate (1 mg/ml protein). Immunoprecipitations were performed overnight at 4uC.Creation of GST-fusion ProteinsUsing the pCaSpeR-4 Roc constructs as template [25], PCR products were made using primers that added EcoR1 sites on either side, and the product was then clone Thu, 12 Jul 2018 00:36:20 UTC en